Journal: Scientific Reports

Article Title: Comparative assessment of commercially available wound gels in ex vivo human skin reveals major differences in immune response-modulatory effects

doi: 10.1038/s41598-022-20997-9

Figure Lengend Snippet: Quantification of cytokines in superficially wounded ex vivo skin treated with commercially available wound gels. Shown are ( a ) IL-6, ( b ) IL-8, ( c ) IL-10, ( d ) MMP2 concentration levels in culture supernatants of 48 h cultured human tape-stripped (TS) skin biopsies topically treated with indicated wound gels as well as a TS untreated control. Samples were analysed in duplicates with an enzyme-linked immunosorbent assay (ELISA). Data is presented as a mean ± standard deviation. A Wilcoxon matched-pairs signed rank test was performed with GraphPad Prism 9.3.1. *p ≤ 0.05; n = 6, female donors, location: abdomen, age range: 20–45 years (panel 1).

Article Snippet: A Wilcoxon matched-pairs signed rank test was performed with GraphPad Prism 9.3.1.

Techniques: Ex Vivo, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific Reports

Article Title: Comparative assessment of commercially available wound gels in ex vivo human skin reveals major differences in immune response-modulatory effects

doi: 10.1038/s41598-022-20997-9

Figure Lengend Snippet: Not all wound gels inactivate MMPs in wounded human ex vivo skin. Shown are activated ( a ) MMP1, MMP2, MMP8, MMP9, MMP12, MMP14 (3 h activation), ( b ) MMP3, MMP10 (24 h activation) concentration levels in culture supernatants of 48 h cultured human tape-stripped (TS) skin biopsies treated with indicated wound gels as well as a TS untreated control. Samples were analysed in duplicates with an MMP activity assay. Relative fluorescence units (RFU) are indicated. Data is presented as a mean ± standard deviation. A Wilcoxon matched-pairs signed rank test was performed with GraphPad Prism 9.3.1. *p ≤ 0.05; n = 6, female donors, location: abdomen, age range: 20–45 years (panel 1).

Article Snippet: A Wilcoxon matched-pairs signed rank test was performed with GraphPad Prism 9.3.1.

Techniques: Ex Vivo, Activation Assay, Concentration Assay, Cell Culture, Activity Assay, Fluorescence, Standard Deviation

Journal: Scientific Reports

Article Title: Comparative assessment of commercially available wound gels in ex vivo human skin reveals major differences in immune response-modulatory effects

doi: 10.1038/s41598-022-20997-9

Figure Lengend Snippet: Numbers of caspase-3 + cells in wounded human ex vivo tissue upon wound gel treatment. Shown is the number of caspase-3 + cells in treated skin biopsies counted within 4 fields of view (FOV) per biopsy (tape-stripped (TS), untreated control and treated with Oct, Pro, Lav, Bet, Act, Mic or Ver) of six donors. FOVs are assessed as technical replicates and data is presented as a mean ± standard deviation. Two-way analysis of variance (ANOVA) was performed using GraphPad Prism 9.3.1. ***p ≤ 0.001, ****p ≤ 0.0001.

Article Snippet: A Wilcoxon matched-pairs signed rank test was performed with GraphPad Prism 9.3.1.

Techniques: Ex Vivo, Standard Deviation